Strain Data Sheet

RBRC10744

Strain Information

Image
BRC No.RBRC10744
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Eno2<tm1(DTA)Hsak>/Ito
Former Common nameNSE-DTA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN.
Strain descriptionCre-dependent DTA expression cassettes was knocked in at the Eno2 locus.
Colony maintenance
References
Genetic dissection of medial habenula-interpeduncular nucleus pathway function in mice.
Kobayashi Y, Sano Y, Vannoni E, Goto H, Suzuki H, Oba A, Kawasaki H, Kanba S, Lipp H P, Murphy N P, Wolfer D P, Itohara S
Front. Behav. Neurosci., 7:17 (2013). 23487260

Innate versus learned odour processing in the mouse olfactory bulb.
Kobayakawa K, Kobayakawa R, Matsumoto H, Oka Y, Imai T, Ikawa M, Okabe M, Ikeda T, Itohara S, Kikusui T, Mori K, Sakano H
Nature, 450(7169) 503-508 (2007). 17989651

Health Report

Examination Date / Room / Rack2024/04/08Room:3-ARack:G
2024/01/09Room:3-ARack:G
2023/10/16Room:3-ARack:G
2023/10/10Room:3-ARack:G
2023/07/10Room:3-ARack:G
2023/04/10Room:3-ARack:G
2023/02/13Room:3-ARack:G
2023/01/10Room:3-ARack:G
2022/10/11Room:3-ARack:G

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DTAdiphtheria toxin A (DT-A)6DTA
Eno2enolase 2, gamma neuronal6Eno2targeted mutation 1, Hitoshi Sakno
Iresinternal ribosomal entry site (EMCV)6
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP sites, mouse phosphoglycerate kinase promoter (Pgk promoter), E.coli neo resistance gene, C.diphtheriae diphtheria toxin A fagment, mouse Eno2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17 (2013).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data