Strain Data Sheet

RBRC10732

Strain Information

Image
BRC No.RBRC10732
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Ntng1<tm2.1Ito>
Former Common nameNtng1 flox
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strain
Appearance
Strain developmentDeveloped by Qi Zhang and Shigeyoshi Itohara at RIKEN. MS12 ES cells derived from C57BL/6NSlc were used. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background.
Strain descriptionNtng1 floxed strain in which a pair of loxP flanks Exon 2.
Colony maintenance
References
Netrin-G1 regulates fear-like and anxiety-like behaviors in dissociable neural circuits.
Zhang Q, Sano C, Masuda A, Ando R, Tanaka M, Itohara S
Sci. Rep., 6:28750 (2016). 27345935

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site3Frt
Ntng1netrin G13Ntng1targeted mutation 2.1, Shigeyoshi Itohara
loxPphage P1 loxP3loxP
loxPphage P1 loxP3loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAP1 phage loxP sites, yeast FRT (flippase recombination target) site, mouse Ntng1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:28750 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Qi Zhang (qi.zhang@a.riken.jp) using the Approval Form.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Qi Zhang and Dr. Shigeyoshi Itohara is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data