Strain Data Sheet

RBRC10725

Strain Information

Image
BRC No.RBRC10725
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Prkcd-cre)PIto
Former Common namePkcd-P-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line P) expressing Cre under the control of Protein Kinase C-delta promoter.
Colony maintenance
References
Netrin-G1 regulates fear-like and anxiety-like behaviors in dissociable neural circuits.
Zhang Q, Sano C, Masuda A, Ando R, Tanaka M, Itohara S
Sci. Rep., 6:28750 (2016). 27345935

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
cre Phage P1 Cre recombinaseUNcre
nls SV40 large T antigen nuclear localization signal (NLS)UNnls mouse Prkcd promoter
SV40 polyAUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse Prkcd genomic DNA, SV40 nuclear location signal(nls), phage P1 Cre recombinase, yeast FRT (flipase recombination target) site, SV40 Transcription termination region
Research applicationCre/loxP system
FLP/frt system
Cre driver
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 6:28750 (2016).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data