Strain Data Sheet

RBRC10725

Information1

Image
BRC No.RBRC10725
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Prkcd-cre)PIto
Former Common namePkcd-P-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line P) expressing Cre under the control of Protein Kinase C-delta promoter.
Colony maintenance
ReferencesSci. Rep., 6:28750 (2016). 27345935

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnlsmouse Prkcd promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAmouse Prkcd genomic DNA, SV40 nuclear location signal(nls), phage P1 Cre recombinase, yeast FRT (flipase recombination target) site, SV40 Transcription termination region
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2016 Jun 27;6:28750.
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data