Strain Data Sheet

RBRC10711

Strain Information

Image
BRC No.RBRC10711
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ntng1<tm1(lacZ)Ito>
Former Common nameNtng1-KO(+LacZ)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells derived from 129P2/ola were used.
Strain descriptionNtng1 (netrin G1) knockout mice. N-LacZ was placed in frame to ATG codon with deletion of 246 bp downstream.
Colony maintenance
References
Axonal netrin-Gs transneuronally determine lamina-specific subdendritic segments.
Nishimura-Akiyoshi S, Niimi K, Nakashiba T, Itohara S
Proc. Natl. Acad. Sci. USA, 104(37):14801-6 (2007). 17785411

Netrin-G/NGL complexes encode functional synaptic diversification.
Matsukawa H, Akiyoshi-Nishimura S, Zhang Q, Lujan R, Yamaguchi K, Goto H, Yaguchi K, Hashikawa T, Sano C, Shigemoto R, Nakashiba T, Itohara S
J. Neurosci., 34(47) 15779-92 (2014). 25411505

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ntng1netrin G13Ntng1targeted mutation 1, Shigeyoshi Itohara
lacZbeta-galactosidase (E. coli)3
loxPphage P1 loxP3loxP
nlsSV40 large T antigen nuclear localization signal (NLS)3nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNASV40 Nuclear laocalozation signal(NLS), E.coli LacZ, pA, P1 phage loxP site, mouse Ntng1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA. 104(37):14801-6 (2007).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data