Strain Data Sheet

RBRC10708

Information1

Image
BRC No.RBRC10708
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-flpe)32Ito
Former Common nameCAG-FLPe
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background.
Strain descriptionTransgenic mice expressing Flpe under the control of CAG promoter (Line 32).
Colony maintenance
ReferencesExp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
flpeyeast flippase recombinaseUNflpeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)UNpuro

Information2

Donor DNAサイトメガロウイルス 初期遺伝子エンハンサー, ニワトリ アクチン遺伝子転写調節領域, 酵母 FLPeリコンビネースコーディング配列, ウサギ beta グロブリン転写調節領域(polyA配列), SV40 Nuclear Localization Signal(NLS), ウシ BGH転写終結配列, ECMV IRES, Streptomyces alboniger puromycin resistance gene
Research applicationFLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 55, 137-141 (2006).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data