Strain Data Sheet

RBRC10707

Information1

Image
BRC No.RBRC10707
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-flpe)16Ito
Former Common nameCAG-FLPe
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background.
Strain descriptionTransgenic mice expressing Flpe under the control of CAG promoter (Line 16).
Colony maintenance
ReferencesExp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack2022/05/23Room:4-ARack:J
2022/03/07Room:4-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone terminator (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
flpeyeast flippase recombinaseUNflpeCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)UNpuro

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 Nuclear Localization Signal(NLS), yeast FLPe cDNA, bovine BGH terminator, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Streptomyces alboniger puromycin resistance gene
Research applicationFLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 55, 137-141 (2006).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data