Strain Data Sheet

RBRC10707

Strain Information

Image
BRC No.RBRC10707
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-flpe)16Ito
Former Common nameCAG-FLPe
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background.
Strain descriptionTransgenic mice expressing Flpe under the control of CAG promoter (Line 16).
Colony maintenance
References
High-efficiency CAG-FLPe deleter mice in C57BL/6J background.
Kanki H, Suzuki H, Itohara S
Exp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone terminator (Bovine)UNGH
Ires internal ribosomal entry site (EMCV)UN
flpe yeast flippase recombinaseUNflpe CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
nls SV40 large T antigen nuclear localization signal (NLS)UNnls
puro puromycin acyltransferase (Streptomyces alboniger)UNpuro

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 Nuclear Localization Signal(NLS), yeast FLPe cDNA, bovine BGH terminator, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), Streptomyces alboniger puromycin resistance gene
Research applicationFLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 55, 137-141 (2006).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data