Strain Data Sheet

RBRC10698

Strain Information

Image
BRC No.RBRC10698
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spp1<tm1.1(GFP)Yham>
Former Common nameSppl-GFP knock-in mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Nagahiro Minato and Yoko Hamazaki, Kyoto University in 2015. Bruce 4 ES cells were used to generate this strain. B6 congenic line.
Strain descriptionEGFP is knocked in at the translation initiation codon of Exon 2 of the Spp1 gene.
Colony maintenance
References
A CD153+CD4+ T follicular cell population with cell-senescence features plays a crucial role in lupus pathogenesis via osteopontin production.
Tahir S, Fukushima Y, Sakamoto K, Sato K, Fujita H, Inoue J, Uede T, Hamazaki Y, Hattori M, Minato N
J. Immunol., 194: 5725-5735 (2015). 25972477

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal5
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFP
Spp1secreted phosphoprotein 15Spp1targeted mutation 1.1, Yoko Hamazaki
loxPphage P1 loxP5loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAAequorea victoria EGFP cDNA, Simian virus SV40 polyA, P1 Bacteriophage LoxP site, mouse Spp1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 194: 5725-5735 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact : Medical Applications Promoting Office, Center for iPS Cell Research and Application, Kyoto University (CiRA) ) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYoko Hamazaki (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data