RBRC10698

Information1

Image
BRC No.RBRC10698
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spp1<tm1.1(GFP)Yham>
Former Common nameSppl-GFP knock-in mouse
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Immunol., 194: 5725-5735 (2015). 25972477

Health Report

Examination Date / Room / Rack2020/04/06Room:3-5Rack:D
2020/02/03Room:3-5Rack:D
2019/12/02Room:3-5Rack:D
2019/10/16Room:3-5Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Spp1secreted phosphoprotein 15Spp1<tm1.1(GFP)Yham>targeted mutation 1.1, Yoko Hamazaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)5EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAAequorea victoria EGFP cDNA, Simian virus SV40 polyA, P1 Bacteriophage LoxP site, mouse Spp1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact : Medical Applications Promoting Office, Center for iPS Cell Research and Application, Kyoto University (CiRA) ) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 194: 5725-5735 (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYoko Hamazaki (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data