Strain Data Sheet

RBRC10697

Information1

Image
BRC No.RBRC10697
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Fabp7-cre/Esr1<*>)#Hoso
Former Common nameBLBP-Cre-ER mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe BLBP promoter (-1547 to -72) (Feng and Heintz, 1995; Anthony et al., 2004) and Cre-ER sequence (Danielian et al., 1998) were obtained by PCR using genomic DNAs of C57 BL/6 and CAGGCre-ER mice (The Jackson Laboratory) (Hayashi and McMahon, 2002), respectively, as templates. The two fragments were subcloned into the upstream region of the beta-globin polyA cassette in the pBI Tet vector (BD Biosciences). BLBP-Cre-ER transgenic mice were generated using standard protocols in 2006.
Strain descriptionCreER expression is driven by the BLBP promoter which is a radial glial cell-specific promoter.
Colony maintenance
ReferencesJ. Neurosci., 31(50):18522-18542 (2011). 22171052

Health Report

Examination Date / Room / Rack2022/07/25Room:3-CRack:F
2022/04/25Room:3-CRack:F
2022/02/14Room:3-CRack:F
2021/11/15Room:3-CRack:F
2021/08/16Room:3-CRack:F
2021/05/17Room:3-CRack:F
2021/02/15Room:3-CRack:F
2020/11/16Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CrePhage P1 Cre recombinaseUNCremouse Fabp7 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Esr1*estrogen receptor 1 (alpha), mouse ER with a mutation G525R)UNEsr1*

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyAUNHBB2

Information2

Donor DNAmouse Fabp7 promoter, P1 phage Cre recombinase, mouse Esr1 cDNA, rabbit beta-globin polyA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31(50):18522-18542 (2011).
DepositorToshihiko Hosoya (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data