Strain Data Sheet
RBRC10697
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10697 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(Fabp7-cre/Esr1<*>)#Hoso |
| Former Common name | BLBP-Cre-ER mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | The BLBP promoter (-1547 to -72) (Feng and Heintz, 1995; Anthony et al., 2004) and Cre-ER sequence (Danielian et al., 1998) were obtained by PCR using genomic DNAs of C57 BL/6 and CAGGCre-ER mice (The Jackson Laboratory) (Hayashi and McMahon, 2002), respectively, as templates. The two fragments were subcloned into the upstream region of the beta-globin polyA cassette in the pBI Tet vector (BD Biosciences). BLBP-Cre-ER transgenic mice were generated using standard protocols in 2006. |
| Strain description | CreER expression is driven by the BLBP promoter which is a radial glial cell-specific promoter. |
| Colony maintenance | |
| References | Periodic organization of a major subtype of pyramidal neurons in neocortical layer V. Maruoka H, Kubota K, Kurokawa R, Tsuruno S, Hosoya T J. Neurosci., 31(50):18522-18542 (2011). 22171052 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/04/22Room:3-CRack:F 2024/01/22Room:3-CRack:F 2023/10/23Room:3-CRack:F 2023/07/26Room:3-CRack:F 2023/07/24Room:3-CRack:F 2023/04/24Room:3-CRack:F 2023/01/23Room:3-CRack:F 2022/10/25Room:3-CRack:F |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Cre | Phage P1 Cre recombinase | UN | Cre | mouse Fabp7 promoter | |||
| Esr1* | estrogen receptor 1 (alpha), mouse ER with a mutation G525R) | UN | Esr1* | ||||
| HBB2 | rabbit beta-globin polyA | UN | HBB2 | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Fabp7 promoter, P1 phage Cre recombinase, mouse Esr1 cDNA, rabbit beta-globin polyA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31(50):18522-18542 (2011). |
| Depositor | Toshihiko Hosoya (RIKEN CBS) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Jager SE, Goodwin G, Chisholm KI, Denk F. In vivo calcium imaging shows that satellite glial cells have increased activity in painful states. Brain Commun 6(2) fcae013(2024) 38638153 |