RBRC10697

Information1

Image
BRC No.RBRC10697
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Fabp7-cre/Esr1<*>)#Hoso
Former Common nameBLBP-Cre-ER mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe BLBP promoter (-1547 to -72) (Feng and Heintz, 1995; Anthony et al., 2004) and Cre-ER sequence (Danielian et al., 1998) were obtained by PCR using genomic DNAs of C57 BL/6 and CAGGCre-ER mice (The Jackson Laboratory) (Hayashi and McMahon, 2002), respectively, as templates. The two fragments were subcloned into the upstream region of the beta-globin polyA cassette in the pBI Tet vector (BD Biosciences). BLBP-Cre-ER transgenic mice were generated using standard protocols in 2006.
Strain descriptionCreER expression is driven by the BLBP promoter which is a radial glial cell-specific promoter.
Colony maintenance
ReferencesJ. Neurosci., 31(50):18522-18542 (2011). 22171052

Health Report

Examination Date / Room / Rack2020/06/01Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
CrePhage P1 Cre recombinaseUNCremouse Fabp7 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Esr1estrogen receptor 1 (alpha)UNEsr1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyAUNHBB2

Information2

Donor DNAmouse Fabp7 promoter, P1 phage Cre recombinase, mouse Esr1 cDNA, rabbit beta-globin polyA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 31(50):18522-18542 (2011).
DepositorToshihiko Hosoya (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

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