Strain Data Sheet

RBRC10695

Information1

Image
BRC No.RBRC10695
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Prkdc<em1Nsas>
Former Common nameB6-Prkdc KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki and Tadashi Okamura in 2018 with the CRISPR/Cas9 technique. C57BL/6JJcl genetic background.
Strain descriptionPrkdc deficient mice in C57BL/6JJcl genetic background.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<em1Nsas>endonuclease-mediated mutation 1, Nobuya Sasaki

Information2

Donor DNAmouse Prkdc
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the content of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data