Strain Data Sheet

RBRC10694

Information1

Image
BRC No.RBRC10694
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Grp<tm1.1(icre)Himo>
Former Common nameGrp-iCre
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hisashi Mori, University of Toyama in 2019. C57BL/6-derived ES cell line RENKA was used. A pgk-Neo cassette was removed by CAG-FLPe (RIKEN BRC, RBRC01834 ). C57BL/6 genetic background.
Strain descriptioniCre was knocked in at the translation initiation site of the gastrin-releasing peptide (Grp) gene.
Colony maintenance
ReferenceseLife 7, e34135 (2018). 29941090

Health Report

Examination Date / Room / Rack2022/06/13Room:3-3Rack:C
2022/03/14Room:3-3Rack:C
2021/12/14Room:3-3Rack:C
2021/09/13Room:3-3Rack:C
2021/06/14Room:3-3Rack:C
2021/03/29Room:3-3Rack:C
2021/01/05Room:3-3Rack:C
2020/10/05Room:3-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grpgastrin releasing peptide18Grp<tm1.1(icre)Himo>targeted mutation 1.1, Hisashi Mori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iCrephage P1 codon improved Cre (iCre)18iCre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Simian virus 40 (SV40) small t-antigen intron18

Information2

Donor DNAMouse Grp gene promoter, Bacteriophage P1 Improved cre recombinase gene (Genesis 32: 19-26, 2002), Simian virus 40 (SV40) small t-antigen intron, Saccharomyces cerevisiae FRT site
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eLife 7, e34135 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application forany patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHisashi Mori (University of Toyama)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data