Strain Data Sheet
RBRC10664
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10664 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C.Cg-Xcr1<tm4(cre)Ksho> |
| Former Common name | XCR1-cre BALB |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2009. |
| Strain description | A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-cre mice were generated by knocking the gene encoding cre recombinase into the murine XCR1 gene locus. In XCR1-cre mice, cre recombinase is expressed secifically in XCR1-expressing cells. |
| Colony maintenance | |
| References | Crucial roles of XCR1-expressing dendritic cells and the XCR1-XCL1 chemokine axis in intestinal immune homeostasis. Ohta T, Sugiyama M, Hemmi H, Yamazaki C, Okura S, Sasaki I, Fukuda Y, Orimo T, Ishii K J, Hoshino K, Ginhoux F, Kaisho T Sci. Rep., 6:23505 (2016). 27005831 |
Health Report | |
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| Examination Date / Room / Rack | 2023/08/28Room:4-BRack:F 2023/05/29Room:4-BRack:F 2023/02/27Room:4-BRack:F 2022/11/29Room:4-BRack:F |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 9 | Frt | ||||
| GH | Growth hormone polyA (Bovine) | 9 | GH | ||||
| Xcr1 | chemokine (C motif) receptor 1 | 9 | Xcr1 | targeted mutation 4, Tsuneyasu Kaisho | |||
| cre | Phage P1 Cre recombinase | 9 | cre | ||||
| neo | neomycin resistance gene (E. coli) | 9 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Bacteriophage P1 Cre, Bos taurus GH polyA, Saccharomyces cerevisiae FRT sites, Herpes simplex virus TK promoter, Polyomavirus Polyomavirus enhancer, Escherichia coli Neomycin resistant gene, Herpes simplex virus TK polyA signal, mouse Xcr1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep. 6:23505 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR. |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |