Strain Data Sheet

RBRC10663

Information1

Image
BRC No.RBRC10663
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xcl1<tm1.2Ksho>
Former Common nameXCL1 KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2009.
Strain descriptionA chemokine, XCL1, is produced by NK cells or activated T cells and functions through a chemokine receptor, XCR1. XCR1 is expressed dominantly in a dendritic cell subset, conventional dendritic cell 1 (cDC1), which shows high ability to support cytotoxic T cell generation. In B6(Cg)-Xcl1tm1.2Ksho mice, the XCL1 gene is disrupted by the insertion of the neomycin resistance gene cassette and the mice carrying the homozygous mutant allele can be used as XCL1 KO mice.
Colony maintenance
ReferencesSci. Rep., 6:23505 (2016). 27005831

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xcl1chemokine (C motif) ligand 11Xcl1<tm1.2Ksho>targeted mutation 1.2, Tsuneyasu Kaisho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Information2

Donor DNABacteriophage P1 loxP site, mouse Xcl1 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep. 6:23505 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data