Strain Data Sheet
RBRC10663
Strain Information | |
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| Image | |
| BRC No. | RBRC10663 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Xcl1<tm1.2Ksho> |
| Former Common name | XCL1 KO |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2009. |
| Strain description | A chemokine, XCL1, is produced by NK cells or activated T cells and functions through a chemokine receptor, XCR1. XCR1 is expressed dominantly in a dendritic cell subset, conventional dendritic cell 1 (cDC1), which shows high ability to support cytotoxic T cell generation. In B6(Cg)-Xcl1tm1.2Ksho mice, the XCL1 gene is disrupted by the insertion of the neomycin resistance gene cassette and the mice carrying the homozygous mutant allele can be used as XCL1 KO mice. |
| Colony maintenance | |
| References | Crucial roles of XCR1-expressing dendritic cells and the XCR1-XCL1 chemokine axis in intestinal immune homeostasis. Ohta T, Sugiyama M, Hemmi H, Yamazaki C, Okura S, Sasaki I, Fukuda Y, Orimo T, Ishii K J, Hoshino K, Ginhoux F, Kaisho T Sci. Rep., 6:23505 (2016). 27005831 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Xcl1 | chemokine (C motif) ligand 1 | 1 | Xcl1 | targeted mutation 1.2, Tsuneyasu Kaisho | |||
| loxP | phage P1 loxP | 1 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP site, mouse Xcl1 genomic DNA |
| Research application | Cre/loxP system Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep. 6:23505 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR. |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |