Strain Data Sheet

RBRC10646

Strain Information

Image
BRC No.RBRC10646
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-Kcnj2/EGFP)20Sleep
Former Common nametetO-Kir2.1-EGFP Tg20
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 20) expressing EGFP-fused Kir2.1 under the control of tetO promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/10/10Room:3-BRack:H
2023/07/10Room:3-BRack:H
2023/04/10Room:3-BRack:H
2023/01/10Room:3-BRack:H
2022/10/11Room:3-BRack:H

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 Intron-polyAUN
  • Andersen-Tawil syndrome(DisGeNET, MedGEN)

  • atrial fibrillation, familial, 9(MedGEN)

  • short QT syndrome type 3(MedGEN)
    • EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
  • Andersen-Tawil syndrome(DisGeNET, MedGEN)

  • atrial fibrillation, familial, 9(MedGEN)

  • short QT syndrome type 3(MedGEN)
    • Kcnj2potassium inwardly-rectifying channel, subfamily J, member 2UNKcnj2E. coli tetO (tetracycline operator)
  • Andersen-Tawil syndrome(DisGeNET, MedGEN)

  • atrial fibrillation, familial, 9(MedGEN)

  • short QT syndrome type 3(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNACMV promoter, E. coli tetO (tetracycline operator), Mus musculus Kir2.1 cDNA, Aequorea victoria GFP cDNA, SV40 Intron-polyA
    Research applicationFluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYu Hayashi (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data