Strain Data Sheet

RBRC10637

Strain Information

Image
BRC No.RBRC10637
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Chat-neo,-tTA)838Sleep
Former Common nameChat-loxP-stop-loxP-tTA Tg838
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 838) expressing tTA under the control of Chat promoter and Cre recombinase.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 Intron-polyAUN
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • loxPphage P1 loxPUNloxP
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • loxPphage P1 loxPUNloxP
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)UN
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAMus musculus Chat gonomic DNA, Bacteriophage P1 loxP sites, phage T7 gb2 promoter, Escherichia coli Neomycin resistance gene, Escherichia coli tTA, SV40 Intron-polyA
    Research applicationCre/loxP system
    Tet system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYu Hayashi (University of Tsukuba)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data