Strain Data Sheet
RBRC10635
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10635 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(Chat-cre)615Sleep |
| Former Common name | Chat-Cre Tg615 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background. |
| Strain description | Transgenic mice (line 615) expressing Cre under the control of Chat promoter. |
| Colony maintenance | |
| References | Enhancement of Haloperidol-Induced Catalepsy by GPR143, an L-Dopa Receptor, in Striatal Cholinergic Interneurons. Masami Arai, Etsuko Suzuki, Satoshi Kitamura, Momoyo Otaki, Kaori Kanai, Miwako Yamasaki, Masahiko Watanabe, Yuki Kambe, Koshi Murata, Yuuki Takada, Tetsu Arisawa, Kenta Kobayashi, Rei Tajika, Tomoyuki Miyazaki, Masahiro Yamaguchi, Michael Lazarus, Yu Hayashi, Shigeyoshi Itohara, Alban de Kerchove d'Exaerde, Hiroyuki Nawa, Ryang Kim, Haruhiko Bito, Toshihiko Momiyama, Daiki Masukawa, Yoshio Goshima J. Neurosci., 44(11):e1504232024 (2024). 38286627 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/01/20Room:3-CRack:ESentinel mouse program 2024/10/21Room:3-CRack:ESentinel mouse program 2024/07/22Room:3-CRack:ESentinel mouse program 2024/04/22Room:3-CRack:ESentinel mouse program 2024/01/29Room:3-CRack:EAdditional tests 2024/01/22Room:3-CRack:ESentinel mouse program 2023/10/23Room:3-CRack:ESentinel mouse program 2023/07/24Room:3-CRack:ESentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Actb | chicken Beta-actin polyA | UN | Actb | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| cre | Phage P1 Cre recombinase | UN | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Mus musculus Chat genomic DNA, SV40 Nuclear Localization Signal(NLS), Bacteriophage P1 Cre, Chicken beta-actin polyA, Saccharomyces cerevisiae FRT site |
| Research application | Cre/loxP system FLP/frt system Cre driver |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 44(11):e1504232024 (2024). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Yu Hayashi and Dr. Shigeyoshi Itohara is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Yu Hayashi (University of Tsukuba) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Arai M, Suzuki E, Kitamura S, Otaki M, Kanai K, Yamasaki M, Watanabe M, Kambe Y, Murata K, Takada Y, Arisawa T, Kobayashi K, Tajika R, Miyazaki T, Yamaguchi M, Lazarus M, Hayashi Y, Itohara S, de Kerchove d'Exaerde A, Nawa H, Kim R, Bito H, Momiyama T, Masukawa D, Goshima Y. Enhancement of Haloperidol-Induced Catalepsy by GPR143, an L-Dopa Receptor, in Striatal Cholinergic Interneurons. J Neurosci 44(11) (2024) 38286627 |