Strain Data Sheet

RBRC10635

Strain Information

Image
BRC No.RBRC10635
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Chat-cre)615Sleep
Former Common nameChat-Cre Tg615
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 615) expressing Cre under the control of Chat promoter.
Colony maintenance
References
J. Neurosci., 44(11):e1504232024 (2024). 38286627

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:E
2024/01/29Room:3-CRack:E
2024/01/22Room:3-CRack:E
2023/10/23Room:3-CRack:E
2023/07/24Room:3-CRack:E
2023/04/24Room:3-CRack:E
2023/01/23Room:3-CRack:E
2022/10/25Room:3-CRack:E

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actbchicken Beta-actin polyAUNActb
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • Frtyeast FRT (flippase recombination target) siteUNFrt
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • crePhage P1 Cre recombinaseUNcre
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)UNnls
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAMus musculus Chat genomic DNA, SV40 Nuclear Localization Signal(NLS), Bacteriophage P1 Cre, Chicken beta-actin polyA, Saccharomyces cerevisiae FRT site
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 44(11):e1504232024 (2024).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Yu Hayashi and Dr. Shigeyoshi Itohara is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYu Hayashi (University of Tsukuba)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data