Strain Data Sheet

RBRC10633

Strain Information

Image
BRC No.RBRC10633
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Chat-cre)609Sleep
Former Common nameChat-Cre Tg609
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 609) expressing Cre under the control of Chat promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:J
2024/01/22Room:3-CRack:J
2023/10/23Room:3-CRack:J
2023/07/24Room:3-CRack:J
2023/04/24Room:3-CRack:J
2023/01/23Room:3-CRack:J
2022/10/25Room:3-CRack:J

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actbchicken Beta-actin polyAUNActb
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • Frtyeast FRT (flippase recombination target) siteUNFrt
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • crePhage P1 Cre recombinaseUNcre
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • loxPphage P1 loxPUNloxP
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)UN
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)UNnls
  • congenital myasthenic syndrome 6(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAMus musculus Chat genomic DNA (BAC clone RP23-246B12), SV40 Nuclear Localization Signal(NLS), Bacteriophage P1 Cre, Chicken Beta-actin polyA, Saccharomyces cerevisiae FRT site, Phage P1 loxP site derived from pBACe3.6 vector, E. coli neomycin resistance gene
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYu Hayashi (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data