Strain Data Sheet

RBRC10626

Information1

Image
BRC No.RBRC10626
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Atoh1-cre/Esr1<*>)676Sleep
Former Common nameAtoh1-CreERT2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing Cre/ERT2 under the control of Atoh1 promoter.
Colony maintenance
ReferencesScience, 350(6263):957-61 (2015). 26494173

Health Report

Examination Date / Room / Rack2023/02/20Room:4-ARack:B
2022/11/21Room:4-ARack:B
2022/08/22Room:4-ARack:B
2022/05/23Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ACTBhuman Beta actinUNACTB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ESR1*human Estrogen receptorUNESR1*

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBBhuman beta globinUNHBB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre

Information2

Donor DNAMus musculus Atoh1 enhancer, Homo sapiens Beta globin genomic DNA, Bacteriophage P1 Cre recombinase, Homo sapiens Estrogen receptor cDNA, Homo sapiens Beta actin genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 350(6263):957-61 (2015).
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data