Strain Data Sheet

RBRC10626

Strain Information

Image
BRC No.RBRC10626
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Atoh1-cre/Esr1<*>)676Sleep
Former Common nameAtoh1-CreERT2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing Cre/ERT2 under the control of Atoh1 promoter.
Colony maintenance
References
Cells of a common developmental origin regulate REM/non-REM sleep and wakefulness in mice.
Hayashi Y, Kashiwagi M, Yasuda K, Ando R, Kanuka M, Sakai K, Itohara S
Science, 350(6263):957-61 (2015). 26494173

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ACTB human Beta actinUNACTB
ESR1* human Estrogen receptorUNESR1*
HBB human beta globinUNHBB
cre Phage P1 Cre recombinaseUNcre

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAMus musculus Atoh1 enhancer, Homo sapiens Beta globin genomic DNA, Bacteriophage P1 Cre recombinase, Homo sapiens Estrogen receptor cDNA, Homo sapiens Beta actin genomic DNA
Research applicationCre/loxP system
Cre driver
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 350(6263):957-61 (2015).
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data