Strain Data Sheet

RBRC10613

Information1

Image
BRC No.RBRC10613
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Usp15<tm1Tchi>
Former Common nameUSP15 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Tomoki Chiba, University of Tsukuba, in 2003. TT2 ES cell lines derived from (C57BL/6 x CBA)F1 were used, and then mice were backcrossed to C57BL/6J for more than 50 generations.
Strain descriptionUsp15 (ubiquitin specific peptidase 15) knock out mice.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Usp15ubiquitin specific peptidase 1510Usp15<tm1Tchi>targeted mutation 1, Tomoki Chiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Polyomavirus SV40 polyA, mouse Usp15 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach
agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTomoki Chiba (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data