Strain Data Sheet
RBRC10611
Strain Information | |
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| Image | |
| BRC No. | RBRC10611 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6N.FVB-Tg(Thy1-GCaMP6, -mCherry)1ShiJna |
| Former Common name | B6N.FVB-Thy1-G6-2A-mCherrv-Tg #38 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Junichi Nakai at Saitama University. Parental strain (RBRC09451) was backcrossed to C57BL/6N for 12 generations at depositor’s lab. |
| Strain description | Transgenic mice expressing GCaMP6 and mCherry under the control of Thy1 promoter. C57BL/6 N congenic line. |
| Colony maintenance | |
| References | Confocal and multiphoton calcium imaging of the enteric nervous system in anesthetized mice. Motegi Y, Sato M, Horiguchi K, Ohkura M, Gengyo-Ando K, Ikegaya Y, Fusamae Y, Hongo Y, Suzuki M, Ogawa K, Takaki M, Nakai J Neurosci. Res., 151:53-60 (2019). 30790590Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J PLoS One, 7, e51286, (2012). 23240011 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| F2A | foot-and-mouth disease virus (FMD) 2A peptide | UN | F2A | ||||
| GCaMP6 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | UN | GCaMP6 | Thy1 promoter | |||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | UN | WPRE | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | UN | mCherry | ||||
| poly A | polyadenylic acid additional signal | UN | poly A | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Thy1 promoter, G-CaMP6(RSET tag, chicken myosin light chain kinase, Jellyfish circularly permutated EGFP cDNA, rat calmodulin cDNA), Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE), mouse polyA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 151:53-60 (2019). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The BIOLOGICAL RESOURCE must not be provided to any third party without DESPOSITOR'S permission. |
| Depositor | Junichi Nakai (Saitama University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |