Strain Data Sheet

RBRC10611

Information1

Image
BRC No.RBRC10611
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N.FVB-Tg(Thy1-GCaMP6, -mCherry)1ShiJna
Former Common nameB6N.FVB-Thy1-G6-2A-mCherrv-Tg #38
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Junichi Nakai at Saitama University. Parental strain (RBRC09451) was backcrossed to C57BL/6N for 12 generations at depositor’s lab.
Strain descriptionTransgenic mice expressing GCaMP6 and mCherry under the control of Thy1 promoter. C57BL/6 N congenic line.
Colony maintenance
ReferencesNeurosci. Res., (2019). 30790590
PLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F2Afoot-and-mouth disease virus (FMD) 2A peptideUNF2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP6Thy1 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Apolyadenylic acid additional signalUNpoly A

Information2

Donor DNAmouse Thy1 promoter, G-CaMP6(RSET tag, chicken myosin light chain kinase, Jellyfish circularly permutated EGFP cDNA, rat calmodulin cDNA), Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE), mouse polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Motegi et al., Confocal and multiphoton calcium imaging of the enteric nervous system in anesthetized mice. Neurosci. Res., (2019).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The BIOLOGICAL RESOURCE must not be provided to any third party without DESPOSITOR'S permission.
DepositorJunichi Nakai (Saitama University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data