Strain Data Sheet

RBRC10606

Strain Information

Image
BRC No.RBRC10606
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc32a1-cre)65Kzy
Former Common nameVGAT-Cre (Tg65), VIAAT-Cre (Tg65), SLC32A1-Cre (Tg65)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J.
Strain descriptionBAC transgenic mice expressing Cre under the control of Slc32a1 (solute carrier family 32 (GABA vesicular transporter), member 1; Vgat) promoter.
Colony maintenance
References
Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated sudden death in a mouse model of Dravet syndrome.
Ogiwara I, Iwasato T, Miyamoto H, Iwata R, Yamagata T, Mazaki E, Yanagawa Y, Tamamaki N, Hensch T K, Itohara S, Yamakawa K
Hum. Mol. Genet., 22(23) 4784-4804 (2013). 23922229

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:H
2024/02/19Room:4-ARack:H
2023/11/20Room:4-ARack:H
2023/08/21Room:4-ARack:H
2023/05/22Room:4-ARack:H
2023/02/20Room:4-ARack:H
2022/11/21Room:4-ARack:H

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
crePhage P1 Cre recombinase2cre

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal brain wave pattern(MP:0004994)

  • abnormal cerebellar molecular layer(MP:0000889)

  • abnormal hippocampus neuron morphology(MP:0009939)

  • abnormal neocortex morphology(MP:0008547)

  • convulsive seizures(MP:0000947)
    • more 4 phenotypes
  • decreased locomotor activity(MP:0001402)

  • postnatal lethality(MP:0002082)

  • postnatal lethality, complete penetrance(MP:0011085)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse VGAT genomic DNA, SV40 large T antigen nuclear localization signal (NLS), Phage P1 Cre, SV40 Transcription termination region, E. coli Kanamycin resistance gene, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-392P11
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 22(23) 4784-4804 (2013).
    DepositorShigeyoshi Itohara (RIKEN CBS)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data