Strain Data Sheet

RBRC10606

Information1

Image
BRC No.RBRC10606
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc32a1-cre)65Kzy
Former Common nameVGAT-Cre (Tg65), VIAAT-Cre (Tg65), SLC32A1-Cre (Tg65)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J.
Strain descriptionBAC transgenic mice expressing Cre under the control of Slc32a1 (solute carrier family 32 (GABA vesicular transporter), member 1; Vgat) promoter.
Colony maintenance
ReferencesHum. Mol. Genet., 22(23) 4784-4804 (2013). 23922229

Health Report

Examination Date / Room / Rack2022/08/22Room:4-ARack:H
2022/05/23Room:4-ARack:H
2022/03/07Room:4-ARack:H
2021/12/06Room:4-ARack:H
2021/09/08Room:4-ARack:H
2021/06/07Room:4-ARack:H
2021/03/22Room:4-ARack:H
2020/12/14Room:4-ARack:H
2020/10/05Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase2cre

Information2

Donor DNAmouse VGAT genomic DNA, SV40 large T antigen nuclear localization signal (NLS), Phage P1 Cre, SV40 Transcription termination region, E. coli Kanamycin resistance gene, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-392P11
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Human Molecular Genetics, 2013, Vol. 22 No. 23 4784-4804In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data