Strain Data Sheet

RBRC10606

Strain Information

Image
BRC No.RBRC10606
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc32a1-cre)65Kzy
Former Common nameVGAT-Cre (Tg65), VIAAT-Cre (Tg65), SLC32A1-Cre (Tg65)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J.
Strain descriptionBAC transgenic mice expressing Cre under the control of Slc32a1 (solute carrier family 32 (GABA vesicular transporter), member 1; Vgat) promoter.
Colony maintenance
References
Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated sudden death in a mouse model of Dravet syndrome.
Ikuo Ogiwara, Takuji Iwasato, Hiroyuki Miyamoto, Ryohei Iwata, Tetsushi Yamagata, Emi Mazaki, Yuchio Yanagawa, Nobuaki Tamamaki, Takao K Hensch, Shigeyoshi Itohara, Kazuhiro Yamakawa
Hum. Mol. Genet., 22(23) 4784-4804 (2013). 23922229

Health Report

Examination Date / Room / Rack2026/06/22Room:3-ARack:DSentinel mouse program
2026/04/06Room:3-ARack:DSentinel mouse program
2026/03/02Room:4-ARack:HSentinel mouse program
2025/12/22Room:4-ARack:HSentinel mouse program
2025/10/14Room:4-ARack:HSentinel mouse program
2025/07/28Room:4-ARack:HSentinel mouse program
2025/05/19Room:4-ARack:HSentinel mouse program
2025/02/17Room:4-ARack:HSentinel mouse program
2024/11/18Room:4-ARack:HSentinel mouse program
2024/08/19Room:4-ARack:HSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cre Phage P1 Cre recombinase2cre

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse VGAT genomic DNA, SV40 large T antigen nuclear localization signal (NLS), Phage P1 Cre, SV40 Transcription termination region, E. coli Kanamycin resistance gene, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-392P11
    Research applicationCre/loxP system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 22(23) 4784-4804 (2013).
    DepositorShigeyoshi Itohara (RIKEN CBS)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Nakashiba T, Sugiyama T, Iwano S, Iwama M, Yoshiki A, Miyawaki A, Abe K.
    Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters.
    Cell Rep Methods 101369(2026) 41916303
    Nakashiba T, Ogoh K, Iwano S, Sugiyama T, Mizuno-Iijima S, Nakashima K, Mizuno S, Sugiyama F, Yoshiki A, Miyawaki A, Abe K.
    Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging.
    Lab Anim (NY) (2023) 37679611