Strain Data Sheet

RBRC10599

Strain Information

Image
BRC No.RBRC10599
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc6a4-GAP/GFP)1Ito
Former Common name5HTT-GAP43-EGFP, TCA-GFP, Sert-GAP43-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background.
Strain descriptionA BAC transgenic mouse in which GAP/GFP is under the control of the Slc6a4 (solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; 5Htt) gene promoter.
Colony maintenance
References
NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates.
Mizuno H, Luo W, Tarusawa E, Saito Y M, Sato T, Yoshimura Y, Itohara S, Iwasato T
Neuron, 82(2) 365-79 (2014). 24685175

Health Report

Examination Date / Room / Rack2024/06/10Room:3-5Rack:C
2024/03/11Room:3-5Rack:C
2023/12/11Room:3-5Rack:C
2023/09/11Room:3-5Rack:C
2023/06/13Room:3-5Rack:C
2023/05/22Room:3-5Rack:C
2023/03/13Room:3-5Rack:C

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40UN
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)
    • EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)
    • Frtyeast FRT (flippase recombination target) siteUNFrt
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)
    • GAP43growth associated protein 43UNGAP43
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse serotonin transporter(5HTTP) genomic DNA, GAP43 palmitoylation signal, jellyfish EGFP cDNA, SV40 polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mizuno et al. Neuron, 82(2) 365-79 (2014).
    DepositorShigeyoshi Itohara (RIKEN CBS)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data