Strain Data Sheet

RBRC10598

Strain Information

Image
BRC No.RBRC10598
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc6a4-cre)208Ito
Former Common name5HTT-Cre, Sert-Cre, SLC6A4-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background.
Strain descriptionA BAC transgenic mouse in which Cre recombinase is under the control of the Slc6a4 (solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; 5Htt) gene promoter.
Colony maintenance
References
Thalamic NMDA receptor function is necessary for patterning of the thalamocortical somatosensory map and for sensorimotor behaviors.
Arakawa H, Suzuki A, Zhao S, Tsytsarev V, Lo F S, Hayashi Y, Itohara S, Iwasato T, Erzurumlu R S
J. Neurosci., 34, 12001-12014 (2014). 25186746

Health Report

Examination Date / Room / Rack2023/11/20Room:4-ARack:H
2023/08/21Room:4-ARack:H
2023/05/22Room:4-ARack:H
2023/02/20Room:4-ARack:H
2022/11/21Room:4-ARack:H

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyAUN
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)
    • Frtyeast FRT (flippase recombination target) siteUNFrt
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)
    • crePhage P1 Cre recombinaseUNcre
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)
    • nlsSV40 large T antigen nuclear localization signal (NLS)UNnls
  • anxiety(MedGEN)

  • obsessive-compulsive disorder(MedGEN)

  • obsolete sudden infant death syndrome(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal excitatory postsynaptic currents(MP:0002910)

  • abnormal primary somatosensory cortex morphology(MP:0000860)

  • abnormal response to tactile stimuli(MP:0005316)

  • abnormal social recognition(MP:0020396)

  • abnormal somatic nervous system physiology(MP:0005423)
    • more 6 phenotypes
  • abnormal somatosensory cortex physiology(MP:0001908)

  • abnormal synaptic transmission(MP:0003635)

  • abnormal thalamus morphology(MP:0000832)

  • disorganized thalamus(MP:0020530)

  • hyporesponsive to tactile stimuli(MP:0005498)

  • short excitatory postsynaptic current decay time(MP:0011274)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Slc6a4 genomic DNA, SV40 nuclear location signal(nls), Phage P1 cre recombinase, SV40 polyA, yeast FRT (flippase recombination target) site
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arakawa et al. J. Neurosci., 34, 12001-12014 (2014).
    DepositorShigeyoshi Itohara (RIKEN CBS)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data