Strain Data Sheet

RBRC10598

Information1

Image
BRC No.RBRC10598
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc6a4-cre)208Ito
Former Common name5HTT-Cre, Sert-Cre, SLC6A4-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background.
Strain descriptionA BAC transgenic mouse in which Cre recombinase is under the control of the Slc6a4 (solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; 5Htt) gene promoter.
Colony maintenance
ReferencesJ. Neurosci., 34, 12001-12014 (2014). 25186746

Health Report

Examination Date / Room / Rack2022/08/22Room:4-ARack:H
2022/05/23Room:4-ARack:H
2022/03/07Room:4-ARack:H
2021/12/06Room:4-ARack:H
2021/09/08Room:4-ARack:H
2021/06/07Room:4-ARack:H
2021/03/22Room:4-ARack:H
2020/12/14Room:4-ARack:H
2020/10/05Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAmouse Slc6a4 genomic DNA, SV40 nuclear location signal(nls), Phage P1 cre recombinase, SV40 polyA, yeast FRT (flippase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arakawa et al. J Neurosci. 2014;34:12001-12014. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data