Strain Data Sheet

RBRC10590

Information1

Image
BRC No.RBRC10590
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(Piwil2-Flag/NLS/Piwil4)#10Nkn
Former Common nameTg-MiliP-FN-Miwi2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toru Nakano, Graduate School of Frontier Biosciences, Osaka University. A DNA construct was injected to fertilized eggs derived from BDF1, and then mice were backcrossed to C57BL/6. Mixed genetic background.
Strain descriptionTransgenic mice (Line 10) expressing Flag-NLS-MIWI2 under the control of mouse mili promoter.
Colony maintenance
ReferencesCell Rep. 2016 Sep 13;16(11):2819-2828. doi: 10.1016/j.celrep.2016.08.027. 27626653

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAGmouse mili promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Piwil4piwi-like RNA-mediated gene silencing 4UNPiwil4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAMouse Mili promoter, FLAG tag, SV40 NLS (nuclear localization signal), Mouse MIWI2 genomic DNA, SV40 polyA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep. 2016 Sep 13;16(11):2819-2828. do1: 10.1016/j.celrep.2016.08.027. PMID: 27626653In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorToru Nakano (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data