RBRC10587

Information1

Image
BRC No.RBRC10587
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Pnldc1<em1Nkn>
Former Common namePnldcl_del-1lnt-Exon3, Pnldc1-KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentCas9 mRNA and each single-guide RNA (sgRNA) transcribed in vitro by the mMESSAGE mMACHINE T7 Ultra Kit (Ambion) were injected into C57BL6 zygotes. Two-cell-stage embryos were transplanted into pseudopregnant mice.
Strain description
Colony maintenance
ReferencesEMBO Rep. 2018 Mar;19(3). pii: e44957. doi: 10.15252/embr.201744957. 29444933

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pnldc1poly(A)-specific ribonuclease (PARN)-like domain containing 117Pnldc1<em1Nkn>endonuclease-mediated mutation 1, Toru Nakano

Information2

Donor DNAマウス Pnldc1
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T. PNLDCl, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 2018 Mar;l9(3). pii: e44957. doi: 10.15252/embr.201744957.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorToru Nakano (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources

BRC mice in Publications

No Data