Strain Data Sheet
RBRC10586
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10586 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6.Cg-Chil3<em1(Venus)Mtka> |
| Former Common name | Ym1-Venus |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masato Tanaka at Tokyo University of Pharmacy and Life Sciences and Masaki Ohmuraya at Kumamoto University in 2014. This strain was generated using KTPU8 ES cells derived from (C57BL/6 x CBA)F1 and mice were backcrossed to C67BL/6J for more than 10 generations. |
| Strain description | Knockin mice in which Venus is inserted at the translation initiation codon of the Chil3 (Ym1) gene. |
| Colony maintenance | |
| References | Emergence of immunoregulatory Ym1(+)Ly6C(hi) monocytes during recovery phase of tissue injury. Ikeda N, Asano K, Kikuchi K, Uchida Y, Ikegami H, Takagi R, Yotsumoto S, Shibuya T, Makino-Okamura C, Fukuyama H, Watanabe T, Ohmuraya M, Araki K, Nishitai G, Tanaka M Sci. Immunol., 3(28)(2018). 30291130 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Chil3 | chitinase-like 3 | 3 | Chil3 | ||||
| Frt | yeast FRT (flippase recombination target) site | 3 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 3 | Frt | ||||
| HBB2 | rabbit beta-globin polyA | 3 | HBB2 | ||||
| Venus | Yellow Fluorescent Protein (Aequorea victoria) | 3 | Venus | ||||
| neo | neomycin resistance gene | 3 | mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | jellyfish Venus cDNA, Rabbit beta-globin polyA, Saccharomyces cerevisiae FRT sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistant gene, mouse Chil3 genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Immunol., 3(28)(2018).The RECIPIENT shall not distribute the biological resource to others without a written consent from the depositor. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER (Masato Tanaka and Gen Nishitai). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Masato Tanaka (Tokyo University of Pharmacy and Life Sciences) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |