Strain Data Sheet

RBRC10583

Information1

Image
BRC No.RBRC10583
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sem1<tm1.1Smta>
Former Common nameSem1 KO
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Jun Hamazaki, The University of Tokyo in 2011. RENKA ES cells derived from C57BL/6N were used. Mice were further backcrossed to C57BL/6N for more than 6 generations.
Strain descriptionSem1 (SEM1, 26S proteasome complex subunit) knockout mice. Exon 2 of the Sem1 gene are deleted. Homozygous mutants are embryonically lethal.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sem1SEM1, 26S proteasome complex subunit6Sem1<tm1.1Smta>targeted mutation 1.1, Shigeo Murata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAPhage P1 loxP site, mouse Sem1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeo Murata (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data