Strain Data Sheet

RBRC10582

Information1

Image
BRC No.RBRC10582
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Adrm1<tm1Smta>
Former Common nameRpn13f/fNeo
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Jun Hamazaki, The University of Tokyo in 2009. RENKA ES cells derived from C57BL/6N were used. Mice were further backcrossed to C57BL/6N for more than 6 generations.
Strain descriptionAdrm1 (adhesion regulating molecule 1, Rpn13 Rpn13) mutant mice. An FRT-flanked neomycin resistance cassette was inserted upstream of exon 3. This fragment and exon 4 were flanked by a pair of loxP.
Colony maintenance
ReferencesPLoS Genet., 11(7): e1005401 (2015). 26222436

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Adrm1adhesion regulating molecule 12Adrm1<tm1Smta>targeted mutation 1, Shigeo Murata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 1oxP sites, Yeast FRT (flippase recombination target) sites, Mouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (E.coli), Pgk polyA, mouse Adrm1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hamazaki J, Hirayama S, Murata S. (2015) Redundant roles of Rpn10 and Rpnl3 in recognition of ubiquitinated proteins and cellular homeostasis . PLoS Genet.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeo Murata (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data