Strain Data Sheet

RBRC10482

Strain Information

Image
BRC No.RBRC10482
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pkm<tm3Ntan>
Former Common namePkmM2
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Nobuhiro Tanuma, Miyagi Cancer Center, in 2012. RENKA ES cells derived from C57BL/6N were used. Mice were backcrossed to C57BL/6N for 7 generations. C57BL/6N genetic background. Y-chromosome is derived from RENKA ES cells.
Strain descriptionAtg13 Knock-in mice expressing PKM2 splicing isoform of the Pkm (pyruvate kinase, muscle) gene. Homozygous mutants are grossly normal and fertile, but exhibit accelerated tumor growth at several experimental tumor models.
Colony maintenance
References
PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.
Mami Morita, Taku Sato, Miyuki Nomura, Yoshimi Sakamoto, Yui Inoue, Ryota Tanaka, Shigemi Ito, Koreyuki Kurosawa, Kazunori Yamaguchi, Yuki Sugiura, Hiroshi Takizaki, Yoji Yamashita, Ryuichi Katakura, Ikuro Sato, Masaaki Kawai, Yoshinori Okada, Hitomi Watanabe, Gen Kondoh, Shoko Matsumoto, Ayako Kishimoto, Miki Obata, Masaki Matsumoto, Tatsuro Fukuhara, Hozumi Motohashi, Makoto Suematsu, Masaaki Komatsu, Keiichi I Nakayama, Toshio Watanabe, Tomoyoshi Soga, Hiroshi Shima, Makoto Maemondo, Nobuhiro Tanuma
Cancer Cell, 33(3): 355-367 (2018). 29533781

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
PKM2 pyruvate kinase, muscle (human)9PKM2
Pkm
MGI:97591		pyruvate kinase, muscle9Pkm<tm3Ntan> targeted mutation 3, Nobuhiro Tanuma
neo neomycin resistance gene (E. coli)9 mouse phosphoglycerate kinase promoter (PGK promoter)
SV40 polyA signal9

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • decreased incidence of induced tumors (MP:0002053)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman PKM1 cDNA, SV40 polyA additional signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pkm genomic DNA
    Research applicationCancer Research
    Metabolism Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Cell, 33(3): 355-367 (2018).
    DepositorNobuhiro Tanuma (Miyagi Cancer Center)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data