Strain Data Sheet

RBRC10482

Information1

Image
BRC No.RBRC10482
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pkm<tm3Ntan>
Former Common namePkmM2
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Nobuhiro Tanuma, Miyagi Cancer Center, in 2012. RENKA ES cells derived from C57BL/6N were used. Mice were backcrossed to C57BL/6N for 7 generations. C57BL/6N genetic background. Y-chromosome is derived from RENKA ES cells.
Strain descriptionAtg13 Knock-in mice expressing PKM2 splicing isoform of the Pkm (pyruvate kinase, muscle) gene. Homozygous mutants are grossly normal and fertile, but exhibit accelerated tumor growth at several experimental tumor models.
Colony maintenance
ReferencesCancer Cell, 33(3): 355-367 (2018). 29533781

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkmpyruvate kinase, muscle9Pkm<tm3Ntan>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PKM2pyruvate kinase, muscle (human)9PKM2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal9

Information2

Donor DNAhuman PKM1 cDNA, SV40 polyA additional signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pkm genomic DNA
Research applicationCancer Research
Metabolism Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mami Morita et al., Cancer Cell, 33(3): 355-367, 2018 (DOI:https//doi.org/10.1016/j.ccell.2018.02.004)
DepositorNobuhiro Tanuma (Miyagi Cancer Center)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data