Strain Data Sheet
RBRC10481
Strain Information | |
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| Image | |
| BRC No. | RBRC10481 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Pkm<tm1Ntan> |
| Former Common name | PkmM1 |
| H-2 Haplotype | |
| ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Nobuhiro Tanuma, Miyagi Cancer Center, in 2012. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were backcrossed to C57BL/6N for 12 generations. C57BL/6N congenic strain. Y-chromosome is derived from C57BL/6NJcl. |
| Strain description | Atg13 Knock-in mice expressing PKM1 splicing isoform of the Pkm (pyruvate kinase, muscle) gene. Homozygous mutants are grossly normal and fertile, but exhibit accelerated tumor growth at several experimental tumor models. |
| Colony maintenance | |
| References | PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama K I, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N Cancer Cell, 33(3): 355-367 (2018). 29533781 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| SV40 polyA signal | 9 | ||||||
| PKM1 | pyruvate kinase, muscle (human) | 9 | PKM1 | ||||
| Pkm | pyruvate kinase, muscle | 9 | Pkm | targeted mutation 1, Nobuhiro Tanuma | |||
| neo | neomycin resistance gene (E. coli) | 9 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | human PKM1 cDNA, SV40 polyA additional signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pkm genomic DNA |
| Research application | Cancer Research Metabolism Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Cell, 33(3): 355-367 (2018). |
| Depositor | Nobuhiro Tanuma (Miyagi Cancer Center) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |