Strain Data Sheet

RBRC10465

Strain Information

Image
BRC No.RBRC10465
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Amy1<em1(luc/mVenus)Matam>
Former Common nameAmy1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naokazu Inoue and Masayuki Sekimata, Fukushima Medical University, and Akiko Sekimata, Yamagata University in 2018. This strain was generated by the CRISPR/Cas9 using fertilized eggs derived from B6D2F1. Mice were further crossed with ICR. Mixed genetic background.
Strain descriptionReporter mouse strain which can monitor Amylase 1 (Amy1) secretion with the NanoLuc (Nluc) and Amy1 expressing cells with mVenus. A DNA construct, Nluc-3xFLAG-T2A-mVenus-BGHpA, was knocked in at downstream of the Amy gene promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Amy1amylase 1, salivary3Amy1endonuclease-mediated mutation, Masayuki Sekimata
FLAG3xFLAG tag (synthetic)3FLAG
GHGrowth hormone polyA3GH
NanoLucluciferase (Oplophorus gracilirostris)3NanoLuc
T2AThosea asigna virus 2A peptide (insect)3T2A
mVenusYellow Fluorescent Protein (Aequorea victoria)3mVenus

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAOplophorus gracilirostris NanoLuc cDNA, synthetic FLAG (3xFLAG tag), Thoseaasigna virus 2A peptide (T2A), Aequorea victoria mVenus (variant of enhanced yellow fluorescent protein) cDNA, Bos taurus GH (Growth hormone polyA), mouse Amy1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorMasayuki Sekimata (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data