Strain Data Sheet

RBRC10464

Strain Information

Image
BRC No.RBRC10464
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Cenpu<tm2.1Tfw>/Yuwa
Former Common nameCenp50floxB6
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Tatsuo Fukagawa, Osaka University, and deposited by Yuichi Wakabayashi, Chiba Cancer Center, in 2018. ES cells derived from 129/Sv were used and then mice were further backcrossed to C57BL/6J for more than 20 generations. C57BL/6J congenic strain. Y choromosome is from C57BL/6J.
Strain descriptionCenpu (centromere protein U, Cenp50) floxed mice. Homozygous null mutants are embryonically lethal. A pair of loxP flanks Exon 4, 5 and 6 of the Cenpu gene.
Colony maintenance
References
The CENP-O complex requirement varies among different cell types.
Kagawa N, Hori T, Hoki Y, Hosoya O, Tsutsui K, Saga Y, Sado T, Fukagawa T
Chromosome Res., 22(3):293-303 (2014). 24481920

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cenpucentromere protein U8Cenputargeted mutation 2.1, Tatsuo Fukagawa
Frtyeast FRT (flippase recombination target) site8Frt
loxPphage P1 loxP8loxP
loxPphage P1 loxP8loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased embryo size(MP:0001698)

  • embryonic lethality between implantation and somite formation(MP:0006205)

  • increased apoptosis(MP:0006042)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Cenpu genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYuichi Wakabayashi (Chiba Cancer Center)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data