Strain Data Sheet

RBRC10460

Information1

Image
BRC No.RBRC10460
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus molossinus
Strain nameMSM-Del(4D4Mit327-D4Mit301)33Arak/Yuwa
Former Common nameMSM-delCh4-33
H-2 Haplotype
ES Cell line
Background strainMSM/Ms
Appearance
Strain developmentDeposited by Yuichi Wakabayashi, Chiba Cancer Center, in 2018. Developed by Kimi Araki, Kumamoto University using ES cells derived from MSM. CRISPR/Cas9 deleted Stmm3 loci (D4Mit327-D4Mit301).
Strain descriptionA mutant (Line 33) on MSM background deleting Stmm3 loci (D4Mit327-D4Mit301). Heterozygous mice are grossly normal. Viability of homozygous mutants is unknown.
Colony maintenance
ReferencesExp. Anim., 63(3):339-48 (2014). 25077764

Health Report

Examination Date / Room / Rack2020/10/05Room:4-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4human Actin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA addition signal4

Information2

Donor DNAhuman Actin promoter, E.coli Neomycin resistant gene, SV40 polyA addition signal
Research applicationCancer Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYuichi Wakabayashi (Chiba Cancer Center)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data