Strain Data Sheet

RBRC10456

Information1

Image
BRC No.RBRC10456
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(MafA-KOr)DIuhw
Former Common nameMafA-Kusabira Orange
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Wataru Nishimura, International University of Health and Wellfare, and Satoru Takahashi, University of Tsukuba. C57BL/6J genetic background.
Strain descriptionBAC transgemic mice in which Kusabira Orange was under the control of MafA gene promoter.
Colony maintenance
ReferencesEndocr. J., 62(1):37-51(2015). 25273397
Islets, 10(3) e1451282 (2018). 29617192

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
KOrKusabira-Orange (Verrillofungia concinna)UNKOrmouse MafA promoter

Information2

Donor DNABacillus amyloliquefaciens SacB promoter, E. coli pUC link, mouse Bacterial artificial chromosome (RP23-94I16), Fungia concinna Kusabira Orange cDNA, yeast FRT (flipase recombination target) site, Bacillus amyloliquefaciens SacB gene*, Phage P1 loxP site*, E. coli Chloramphenicol resistance gene*, E. coli parA gene, E. coli parB gene(* Not detected by PCR. E. coli parA and parB genes were not tested.)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Endocr. J., 62(1):37-51(2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorWataru Nishimura (International University of Health and Welfare)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data