RBRC10449

Information1

Image
BRC No.RBRC10449
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Hacd1<tm1Akih>
Former Common nameHacd1 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Biol. Chem., 292, 15538-15551 (2017). 28784662

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hacd13-hydroxyacyl-CoA dehydratase 12Hacd1<tm1Akih>targeted mutation 1, Akio Kihara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2E. coli EM7 promoter, Mouse Phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNABacteriophage P1 LoxP sites, Saccharomyces cerevisiae FRT sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Hacd1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkio Kihara (Hokkaido University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data