Strain Data Sheet

RBRC10443

Strain Information

Image
BRC No.RBRC10443
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Clgn-Izumo1/EYFP1-155<*>F46L/V150A/M153T, Clgn-Izumo1/EYFP156-239<*>A206K)1Nino
Former Common nameIzumo1-BiFC TG
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naokazu Inoue, Fukushima Medical University in 2018. B6D2 genetic background.
Strain descriptionTransgenic mice expressing Izumo1 proteins fused with EYFP (1-155) and EYFP (156-239) under the control of Clgn gene promoter. Both EYFP fragments are separately fused with Izumo1 proteins.
Colony maintenance
References
Monitoring dimeric status of IZUMO1 during the acrosome reaction in living spermatozoon.
Inoue N, Wada I
Cell Cycle, 17(11):1279-1285 (2018). 29954238

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP
HBB2rabbit beta-globin polyAUNHBB2
Izumo1izumo sperm-egg fusion 1UNIzumo1mouse Clgn promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Calmegin promoter, mouse Izumo1 cDNA, jellyfish Eyfp<*> F46L/V150A/M153T/A206K cDNA, rabbit beta-globin polyA signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorNaokazu Inoue (Fukushima Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data