Strain Data Sheet

RBRC10428

Information1

Image
BRC No.RBRC10428
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xkr8<tm1.1Osa>
Former Common nameXkr8 flox/flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Shigekazu Ngata and Jun Suzuki at Osaka University in 2013. Bruce 4 ES cell line was used. A pair of loxP flanks Exon 3 of the Xkr8 gene. Neomycin resistance gene was removed by crossing CAG-Flp mice.
Strain descriptionXkr8 (X-linked Kx blood group related 8) floxed mice.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 115(9):2132-2137 (2018). 29440417
Science, 341, 403-406 (2013). 23845944

Health Report

Examination Date / Room / Rack2022/07/11Room:3-BRack:G
2022/04/11Room:3-BRack:G
2022/01/24Room:3-BRack:G
2021/10/26Room:3-BRack:G
2021/07/26Room:3-BRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xkr8X-linked Kx blood group related 84Xkr8<tm1.1Osa>targeted insertion 1.1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Xkr8 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 341, 403-406 (2013).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data