BRC No.RBRC10414
SpeciesMus musculus
Strain nameC57BL/6N-Elovl3<em1Sato>/F0-2b
Former Common nameElovl3 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Strain developmentC57BL/6NCrSlcから得られた受精卵を用いてCRISPR/Cas9システムにより作製国際電気通信基礎技術研究所 佐藤匠徳特別研究所、佐藤匠徳、2015年C57BL/6N系統、C57BL/6NCrSlcから得られた受精卵を用いて作製。F1世代のヘテロ接合体とC57BL/6NCrSlcのIVFによって得られた2細胞期胚を保存。自然交配可能。
Strain descriptionCRISPR/Cas9 システムによって作製したElovl3(elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3)ノックアウトマウス。エクソン2内のフレームシフトにより翻訳が停止する。
Colony maintenance

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Elovl3elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 319Elovl3<em1Sato>endonuclease mediated mutation 1, Thomas N. Sato


Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorThomas N. Sato (Advanced Telecommunications Research Institute International (ATR))
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data