RBRC10394

Information1

Image
BRC No.RBRC10394
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Nmrk2<em3Sato>/F0-8b
Former Common nameNmrk2 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentC57BL/6NCrSlcから得られた受精卵を用いてCRISPR/Cas9システムにより作製国際電気通信基礎技術研究所 佐藤匠徳特別研究所、佐藤匠徳、2015年C57BL/6N系統、C57BL/6NCrSlcから得られた受精卵を用いて作製。F1世代のヘテロ接合体とC57BL/6NCrSlcのIVFによって得られた2細胞期胚を保存。F0-7b型のみF2世代のヘテロ接合体とF3世代のヘテロ接合体のIVFによって得られた2細胞期胚の保存あり。ヘテロ接合体同士の交配で系統維持可能。
Strain descriptionCRISPR/Cas9 システムによって作製したNmrk2(nicotinamide riboside kinase 2)ノックアウトマウス。いづれもエクソン2内のフレームシフトにより翻訳が停止する。
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nmrk2nicotinamide riboside kinase 210Nmrk2<em3Sato>endonuclease mediated mutation 3, Thomas N. Sato

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorThomas N. Sato (Advanced Telecommunications Research Institute International (ATR))
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data