RBRC10392

Information1

Image
BRC No.RBRC10392
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ebf3<tm1(cre/ERT2)Tng>
Former Common nameEbf3-CreERT2
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strainC57BL/6NCrSlc
Appearance
Strain development
Strain descriptionEbf3-CreERT knock-in mice. These mice have a tamoxifen-inducible cre (CreERT2) at the Ebf3 locus. IRES-CreERT2 and FRT-PGKpro-neo-pA-FRT cassettes were inserted into the 3' UTR of exon 16 of the Ebf3 locus.
Colony maintenance
ReferencesGenes Dev., 32, 359-372 (2018). 29563184

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ebf3early B cell factor 37Ebf3<tm1(cre/ERT2)Tng>

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)7ERT2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase7cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEncephalomyocarditis virus Internal ribosomal entry site, Bacteriophage P1 Cre, Human ERT2 cDNA, Yeast Frt sites, Mouse PGK promoter, E. coli Neomycin resistant gene, Mouse PolyA signal, mouse Ebf3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takashi Nagasawa) and enter into a Material Transfer Agreement with Osaka University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTakashi Nagasawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data