RBRC10390

Information1

Image
BRC No.RBRC10390
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Actb<tm3.1(tetO-GCaMP6)Kftnk>Tg(Pvalb-tTA)1Kftnk
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 109(50), 20720-20725 (2012). 23185019

Health Report

Examination Date / Room / Rack2019/09/30Room:4-ARack:D
2019/07/29Room:4-ARack:D
2019/05/27Room:4-ARack:D
2019/03/25Room:4-ARack:D
2019/01/28Room:4-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Actbactin, beta5Actb<tm1(tetO-YCnano50)Kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA5GCaMP6E. coli tet operator, cytomegalovirus CMV promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse parvalbumin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAP1 Phage loxP sequence, yeast FRT (flipase recombination target) site, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA, mouse Pvalb genomic DNA (BAC clone RP24-450L20), E. coli tet repressor (tetR), herpes simplex virus (HSV)VP16 activation domain, SV40 polyA additional signal
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Proc Natl Acad Sci U S A., 109(50), 20720-20725 (2012)). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT should cite Kenji F. Tanaka as co-author in the first paper.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data