Strain Data Sheet

RBRC10389

Information1

Image
BRC No.RBRC10389
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameICR.Cg-Kiss1<tm1.1Nurep>
Former Common nameKiss1-floxed (#96)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainCrlj:CD1(ICR)
Appearance
Strain developmentDeposited by Hiroko Tsukamura, Nagoya University. TT2 (CBAﾗC57BL/6) ES cells were used and then mice were crossed with ICR. Mixed genetic background. A neo fragment was deleted from this allele.
Strain descriptionKiss1 floxed mice. A pair of loxP sites flank Exon 3 of the Kiss1 gene.
Colony maintenance
ReferencesJ. Reprod. Dev., 66(4):359-367 (2020). 32307336
J. Reprod. Dev., 66(4):369-375 (2020). 32336702

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kiss1KiSS-1 metastasis-suppressor1Kiss1<tm1.1Nurep>targeted mutation 1.1, Lab. of Reproductive Science, Nagoya University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP1loxP

Information2

Donor DNAphage P1 loxP sites, mouse Kiss1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Reprod. Dev., 66(4):359-367 (2020). J. Reprod. Dev., 66(4):369-375 (2020).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from either Prof. Hiroko Tsukamura or Dr. Yoshihisa Uenoyama using the Approval Form. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of its use with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorHiroko Tsukamura (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data