Strain Information | |
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Image | |
BRC No. | RBRC10386 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Pld2<tm1Yaka> |
Former Common name | PLD2-KO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yasunori Kanaho and Takeaki Yokozeki, University of Tsukuba in 2006. TT2 ES cells derived from (C57BL/6NCrlj x CBA/JNCrlj)F1 was used, and then mice were backcrossed to C57BL/6J over 10 generations. |
Strain description | Pld2 (phospholipase D2) knockout mice. A floxed Neo fragement replaced Exon 14 and 15. |
Colony maintenance | |
References | Molecular mechanisms of N-formyl-methionyl-leucyl-phenylalanine-induced superoxide generation and degranulation in mouse neutrophils: phospholipase D is dispensable. Sato T, Hongu T, Sakamoto M, Funakoshi Y, Kanaho Y Mol. Cell Biol., 33(1) 136-145 (2013). 23109426Physiological function of phospholipase D2 in anti-tumor immunity: regulation of CD8(+) T lymphocyte proliferation. Ngo Thai Bich V, Hongu T, Miura Y, Katagiri N, Ohbayashi N, Yamashita-Kanemaru Y, Shibuya A, Funakoshi Y, Kanaho Y Sci. Rep., 8(1) 6283 (2018). 29674728 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Pld2 MGI:892877 | phospholipase D2 | 11 | Pld2<tm1Yaka> MGI:5468333 | targeted mutation 1, Yasunori Kanaho | |||
loxP | phage P1 loxP | 11 | loxP | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 11 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, herpes simplex virus-1 thymidine kinase promoter (HSV tk promoter), E. coli neo, Herpes simplex virus-1 Thymidine kinase poly(A)signal, mouse Pld2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 33(1), 136-145 (2013), Sci. Rep., 8(1) 6283 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasunori Kanaho (University of Tsukuba) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |