Strain Data Sheet

RBRC10386

Information1

Image
BRC No.RBRC10386
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pld2<tm1Yaka>
Former Common namePLD2-KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Takeaki Yokozeki, University of Tsukuba in 2006. TT2 ES cells derived from (C57BL/6NCrlj x CBA/JNCrlj)F1 was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain descriptionPld2 (phospholipase D2) knockout mice. A floxed Neo fragement replaced Exon 14 and 15.
Colony maintenance
ReferencesSci. Rep., 8(1) 6283 (2018). 29674728
Mol. Cell Biol., 33(1) 136-145 (2013). 23109426

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pld2phospholipase D211Pld2<tm1Yaka>targeted mutation 1, Yasunori Kanaho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAphage P1 loxP sites, herpes simplex virus-1 thymidine kinase promoter (HSV tk promoter), E. coli neo, Herpes simplex virus-1 Thymidine kinase poly(A)signal, mouse Pld2 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 33(1), 136-145 (2013), Sci. Rep., 8(1) 6283 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasunori Kanaho (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data