Strain Information | |
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Image | |
BRC No. | RBRC10386 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Pld2<tm1Yaka> |
Former Common name | PLD2-KO |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yasunori Kanaho and Takeaki Yokozeki, University of Tsukuba in 2006. TT2 ES cells derived from (C57BL/6NCrlj x CBA/JNCrlj)F1 was used, and then mice were backcrossed to C57BL/6J over 10 generations. |
Strain description | Pld2 (phospholipase D2) knockout mice. A floxed Neo fragement replaced Exon 14 and 15. |
Colony maintenance | |
References | Sci. Rep., 8(1) 6283 (2018). 29674728 Mol. Cell Biol., 33(1) 136-145 (2013). 23109426 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, herpes simplex virus-1 thymidine kinase promoter (HSV tk promoter), E. coli neo, Herpes simplex virus-1 Thymidine kinase poly(A)signal, mouse Pld2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 33(1), 136-145 (2013), Sci. Rep., 8(1) 6283 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasunori Kanaho (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |