Strain Data Sheet

RBRC10385

Information1

Image
BRC No.RBRC10385
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Pld1<tm1.1Mafr>
Former Common namePLD1-KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Tsunaki Hongu, University of Tsukuba in 2006. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain descriptionPld1 (phospholipase D1) knockout mice. A loxP site was inserted upstream of Exon 13. An FRT-flanked neo fragment with a 3' loxP site was inserted downstream of exon 13. The neo fragment and Exon 13 were removed by crossing with a Ayu1-Cre mouse.
Colony maintenance
ReferencesSci. Signal., 5(249) ra79 (2012). 23131846
Mol. Cell Biol., 33(1) 136-145 (2013). 23109426

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pld1phospholipase D13Pld1<tm1.1Mafr>targeted mutation 1.1, Michael Frohman

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Information2

Donor DNAphage P1 loxP site, mouse Pld1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Signal., 5(249) ra79 (2012). Mol. Cell Biol., 33(1) 136-145 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasunori Kanaho (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data