Strain Data Sheet

RBRC10384

Information1

Image
BRC No.RBRC10384
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pip5k1b<tm1Tssk>
Former Common namePip5k1b-KO
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Takehiko Sasaki, Tokyo Metropolitan Institute of Medical Science in 2004. E14K ES cells derived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain descriptionPip5k1b (phosphatidylinositol-4-phosphate 5-kinase, type 1 beta) knockout mice. A neo fragment replaced Exon 3 of the Pip5k1b gene. Homozygous mutants exhibit enhanced passive cutaneous and systemic anaphylaxis.
Colony maintenance
ReferencesJ. Exp. Med., 201(6) 859-870 (2005). 15767368

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pip5k1bphosphatidylinositol-4-phosphate 5-kinase, type 1 beta19Pip5k1b<tm1Tssk>targeted mutation 1, Takehiko Sasaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly(A) signal, mouse Pip5k1b genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 201(6) 859-870 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasunori Kanaho (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data