Strain Data Sheet

RBRC10384

Strain Information

Image
BRC No.RBRC10384
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pip5k1b<tm1Tssk>
Former Common namePip5k1b-KO
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Takehiko Sasaki, Tokyo Metropolitan Institute of Medical Science in 2004. E14K ES cells derived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain descriptionPip5k1b (phosphatidylinositol-4-phosphate 5-kinase, type 1 beta) knockout mice. A neo fragment replaced Exon 3 of the Pip5k1b gene. Homozygous mutants exhibit enhanced passive cutaneous and systemic anaphylaxis.
Colony maintenance
References
Regulation of anaphylactic responses by phosphatidylinositol phosphate kinase type I {alpha}.
Sasaki J, Sasaki T, Yamazaki M, Matsuoka K, Taya C, Shitara H, Takasuga S, Nishio M, Mizuno K, Wada T, Miyazaki H, Watanabe H, Iizuka R, Kubo S, Murata S, Chiba T, Maehama T, Hamada K, Kishimoto H, Frohman M A, Tanaka K, Penninger J M, Yonekawa H, Suzuki A, Kanaho Y
J. Exp. Med., 201(6) 859-870 (2005). 15767368

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pip5k1bphosphatidylinositol-4-phosphate 5-kinase, type 1 beta19Pip5k1btargeted mutation 1, Takehiko Sasaki
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • impaired fertilization(MP:0000242)

  • reduced male fertility(MP:0001922)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly(A) signal, mouse Pip5k1b genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 201(6) 859-870 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYasunori Kanaho (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data