Strain Data Sheet

RBRC10383

Information1

Image
BRC No.RBRC10383
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pip5k1a<tm1Tba>
Former Common name
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Takamitsu Unoki, University of Tsukuba in 2005. E14 ES cells derived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain description)Pip5k1a (phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha) knockout mice. A floxed neo fragment replaced Exon 5 of the Pip5k1a gene. Homozygous males are infertile.
Colony maintenance
ReferencesSci. Rep., 7(1) 9438 (2017). 28842595
Biol. Reprod., 86(5) 136, 1-12 (2012). 22321832

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pip5k1aphosphatidylinositol-4-phosphate 5-kinase, type 1 alpha3Pip5k1a<tm1Tba>targeted mutation 1, Tadashi Baba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Pip5k1a genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 86(5) 136, 1-12 (2012). Sci. Rep., 7(1) 9438 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasunori Kanaho (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data