Strain Data Sheet

RBRC10382

Information1

Image
BRC No.RBRC10382
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Arf6<tm2.1Yaka>
Former Common nameArf6-flox
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Tunaki Hongu, University of Tsukuba in 2008. TT2 ES cells dereived from (C57BL/6NCrlj x CBA/JNCrlj)F1 was used, and then mice were backcrossed to C57BL/6J for more than 10 generations.
Strain descriptionArf6 floxed mice. A pair of loxP sites flank Exon 1 and 2 of the Arf6 gene. Homozygous floxed mice show no gross abnormality.
Colony maintenance
ReferencesNat. Commun., 5, 4744 (2014). 25144208
Nat. Commun., 6, 7925 (2015). 26239146
Sci. Rep., 7, 46649 (2017). 28429746

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Arf6ADP-ribosylation factor12Arf6<tm2.1Yaka>targeted mutation 2.1, Yasunori Kanaho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Information2

Donor DNAphage P1 loxP sites, yeast(S.crevisiae) FRT (flippase recombination target) site, mouse Arf6 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 5, 4744 (2014). Nat. Commun., 6, 7925 (2015). Sci. Rep., 7, 46649 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasunori Kanaho (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data