Strain Data Sheet


Strain Information

BRC No.RBRC10381
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Arf6<tm1Yaka>
Former Common nameArf-6
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strain
Strain developmentDeveloped by Yasunori Kanaho and Teruhiko Suzuki, University of Tsukuba in 2004. A neomycin resistance gene replaced a genomic region containing the translation initiation codon of the Arf6 gene. E14K ES cells dereived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain descriptionArf6 knockout mice. Homozygous mutants are embryonically lethal due to impaired liver development.
Colony maintenance
ReferencesMol. Cell Biol., 26(16), 6149-56 (2006). 16880525

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Arf6ADP-ribosylation factor 612Arf6<tm1Yaka>targeted mutation 1, Yasunori Kanaho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly(A) signal, mouse Arf6 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 26(16), 6149-56. (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasunori Kanaho (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data