Strain Data Sheet

RBRC10371

Strain Information

Image
BRC No.RBRC10371
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ppp1r14a<em1Mhori>
Former Common nameCPI-17 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Masatoshi Hori, The University of Tokyo. With the CRISPR/Cas9, 76 bp DNA fragment of the Ppp1r14a gene was deleted. C57BL/6J genetic background. Homozygous mutants exhibit deficits in smooth muscle contraction and blood pressure.
Strain descriptionPpp1r14a mutant mice. This mutant has 76 bp deletion of the Ppp1r14a gene.
Colony maintenance
References
The essential role of phospho-T38 CPI-17 in the maintenance of physiological blood pressure using genetically modified mice.
Yang Q, Fujii W, Kaji N, Kakuta S, Kada K, Kuwahara M, Tsubone H, Ozaki H, Hori M
FASEB J. 32(4), 2095-2109 (2018). 29229685

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ppp1r14a
MGI:1931139		protein phosphatase 1, regulatory inhibitor subunit 14A7Ppp1r14a<em1Mhori> endonuclease-mediated mutation 1, Masatoshi Hori

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • decreased systemic arterial blood pressure (MP:0002843)

  • increased heart rate (MP:0002626)

  • increased systemic arterial systolic blood pressure (MP:0006144)
    • Detailed phenotype data

    Ordering Information

    Donor DNAStreptococcus pyogenes cas9 (mRNA), mouse Ppp1r14a genomic DNA, sgRNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 32(4), 2095-2109 (2018).
    DepositorMasatoshi Hori (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data