Strain Data Sheet

RBRC10371

Information1

Image
BRC No.RBRC10371
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ppp1r14a<em1Mhori>
Former Common nameCPI-17 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Masatoshi Hori, The University of Tokyo. With the CRISPR/Cas9, 76 bp DNA fragment of the Ppp1r14a gene was deleted. C57BL/6J genetic background. Homozygous mutants exhibit deficits in smooth muscle contraction and blood pressure.
Strain descriptionPpp1r14a mutant mice. This mutant has 76 bp deletion of the Ppp1r14a gene.
Colony maintenance
ReferencesFASEB J. 32(4), 2095-2109 (2018). 29229685

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ppp1r14aprotein phosphatase 1, regulatory inhibitor subunit 14A7Ppp1r14a<em1Mhori>

Information2

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 32(4), 2095-2109 (2018).
DepositorMasatoshi Hori (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data