Strain Data Sheet

RBRC10368

Strain Information

Image
BRC No.RBRC10368
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pin1<tm1Tuc>
Former Common namePin1-KO mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takashi Uchida, Graduate School and Faculty of Agricultural Science, Tohoku University. E14 ES derived from 129P2/OlaHsd cells were used, and then mice were backcrossed to C57BL/6J over 13 generations.
Strain descriptionPin1, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 knockout mice. A neomycin resistance cassette replaced exons 1-4.
Colony maintenance
References
Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes.
Liou Y C, Ryo A, Huang H K, Lu P J, Bronson R, Fujimori F, Uchida T, Hunter T, Lu K P
Proc. Natl. Acad. Sci. USA, 99, 1335-1340 (2002). 11805292

Mice lacking Pin1 develop normally, but are defective in entering cell cycle from G(0) arrest.
Fujimori F, Takahashi K, Uchida C, Uchida T
Biochem. Biophys. Res. Commun., 265, 658-663 (1999). 10600477

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pin1peptidyl-prolyl cis/trans isomerase, NIMA-interacting 19Pin1targeted mutation 1, Takafumi
loxPphage P1 loxP9loxP
loxPphage P1 loxP9loxP
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mammary gland growth during pregnancy(MP:0006269)

  • abnormal ovarian follicle number(MP:0008871)

  • abnormal platelet cell number(MP:0011898)

  • abnormal spermatogenesis(MP:0001156)

  • abnormal spermatogonia morphology(MP:0006378)
    • more 13 phenotypes
  • amyloid beta deposits(MP:0003329)

  • azoospermia(MP:0005159)

  • decreased body weight(MP:0001262)

  • decreased litter size(MP:0001935)

  • decreased primordial germ cell number(MP:0008392)

  • decreased primordial germ cell proliferation(MP:0030934)

  • decreased testis weight(MP:0004852)

  • increased megakaryocyte cell number(MP:0008254)

  • reduced female fertility(MP:0001923)

  • reduced male fertility(MP:0001922)

  • retina degeneration(MP:0001326)

  • seminiferous tubule degeneration(MP:0001154)

  • testicular atrophy(MP:0003205)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pin1 genomic DNA
    Research applicationCancer Research
    Cardiovascular Research
    Cre/loxP system
    Diabetes and Obesity Research
    Immunology and Inflammation Research
    covid19_progression
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 265, 658-663 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakafumi Uchida (Tohoku University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data