Strain Data Sheet

RBRC10360

Information1

Image
BRC No.RBRC10360
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Il22ra2<tm1.1Ksat>
Former Common nameIl22ra2-/- mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuaki Sato, Miyazaki University in 2018. JN/2 ES cells derived from B6 were used, and then mice were backcrossed to C57BL/6J over 12 generations. C57BL/6 congenic strain. Stop cassette was knocked in at Exon 1 of the Il22ra2 gene.
Strain descriptionIl22ra2(interleukin 22 receptor, alpha 2) knockout mice.
Colony maintenance
ReferencesFront. Immunol., 9, 1418 (2018). 29977242

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il22ra2interleukin 22 receptor, alpha 210Il22ra2<tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)10EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)10HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
herpes simplex virus thymidine kinase (HSV TK) polyA10

Information2

Donor DNASTOP cassette (human HBEGF cDNA, jellyfish EGFP cDNA, herpes simplex virus thymidine kinase (HSV TK) poly A), Phage P1 loxP site, mouse Il22ra2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front Immunol. 2018 Jun 21, 9:1418. doi, 10.3389/fimmu.2018.01418Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Katsuaki Sato) using the Approval Form. The Recipient acknowledges that RIKEN (and/or its original provider) reserves title to the Material. The Recipient will not transfer the Material to any scientists. The Recipient will not publish or present Research result without obtaining prior permission from the DEVELOPER (Dr. Katsuaki Sato). The Recipient will send Dr. Katsuaki Sato any abstract of submission, oral or written presentation, and/or copy of draft of paper or research article which is resulted from the Research at least 30 days prior to the submission. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEVELOPER (Dr. Katsuaki Sato).
DepositorKatsuaki Sato (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data